ELISA - Enzyme Linked Immunosorbent Assay
eBook - PDF
Available until 10 Nov |Learn more

ELISA - Enzyme Linked Immunosorbent Assay

reliable and cheap method for the detection of food allergens

Amjad Iqbal

  1. 80 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF
Available until 10 Nov |Learn more

ELISA - Enzyme Linked Immunosorbent Assay

reliable and cheap method for the detection of food allergens

Amjad Iqbal

Book details
Table of contents
Citations

About This Book

In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.

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Information

Publisher
LAP LAMBERT Academic Publishing
Year
2012
ISBN
9783848436637
Edition
1
Topic
Biological Sciences
Subtopic
Horticulture
Index
Biological Sciences

Table of contents

    Citation styles for ELISA - Enzyme Linked Immunosorbent Assay

    APA 6 Citation

    Iqbal, A. (2012). ELISA - Enzyme Linked Immunosorbent Assay (1st ed.). LAP LAMBERT Academic Publishing. Retrieved from https://www.perlego.com/book/3359849 (Original work published 2012)

    Chicago Citation

    Iqbal, Amjad. (2012) 2012. ELISA - Enzyme Linked Immunosorbent Assay. 1st ed. LAP LAMBERT Academic Publishing. https://www.perlego.com/book/3359849.

    Harvard Citation

    Iqbal, A. (2012) ELISA - Enzyme Linked Immunosorbent Assay. 1st edn. LAP LAMBERT Academic Publishing. Available at: https://www.perlego.com/book/3359849 (Accessed: 8 July 2024).

    MLA 7 Citation

    Iqbal, Amjad. ELISA - Enzyme Linked Immunosorbent Assay. 1st ed. LAP LAMBERT Academic Publishing, 2012. Web. 8 July 2024.